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A research team led by Drs. Juyeon Jung and Eun-Kyung Lim at the Hazards Monitoring Bionano Research Center, Korea Research Institute of Bioscience and Biotechnology developed a lateral flow biosensor based on an oseltamivir derivative (oseltamivir hexylthiol;OHT) for point of care (POC) diagnostics that can be used to detect Tamiflu-resistant influenza virus rapidly.
In order to develop this diagnostics of using an oseltamivir-based molecule, OHT-modified gold nanoparticles (OHT-GNPs) was first developed as a colorimetric probe. In the presence of Tamiflu-resistant virus, a colorimetric change from deep red to purple was observed due to OHT-GNPs aggregation driven by strong interaction between OHT and neuraminidase (NA). Further, lateral flow assays (LFAs) were fabricated by using OHT-GNPs as POC devices for investigating the specific interaction between OHT and Tamiflu-resistant virus. This research proves that various OHT-based diagnostic assays could be developed and facilitate the development of POC diagnostic platforms for simple detection of Tamiflu-resistant virus.
Further details of the research can be found at Scientific Reports online published on August, 29, 2018, https://www.nature.com/articles/s41598-018-31311-x