Current page location

Biomedical Genomics Research Center

Biomedical Genomics Research Center
Objectives
Our goal is to establish world-class genomics-based technology platforms and to apply them to biomedical research programs. This will achieve high-throughput identification and global function analysis of the genes associated with diseases most prevalent in the Korean population, such as colon, stomach and liver cancers. We also conduct functional and chemical genomics research to discover validated targets and biomarkers for the development of effective diagnostics and therapeutics.
Researchers
    Click on the name
researchers and research statement
name research fields
Nam-Soon Kim
/ Director
(Concurrent Position)
  • Identification and functional study of therapeutic novel targets related to cancers and large-scale collection of full-length human cDNAs
Yong-Kyung Choe
/ Principal Researcher
  • Mechanism of cisplatin-resistance
Dong-Soo Im
/ Principal Researcher
  • Identification and validation of target for cancer therapy
Yong Sung Kim
/ Principal Researcher
  • Epigenetics and epigenomics study in human gastric and colorectal cancers
Mi Sun Won
/ Principal Researcher
  • Functional validation of candidate target genes and development of anticancer drugs by chemical screening and study of modes of action
Hee Gu Lee
/ Principal Researcher
  • Production and application of antibodies for functional analysis of cancer related genes
Dong Cho Han
/ Principal Researcher
  • Study of cancer cell migration and metastasis using chemical biology
Kyung-Sook Chung
/ Principal Researcher
  • Development of anticancer drugs by chemical screening and study of modes of action
Jae Wha Kim
/ Principal Researcher
  • Efficacy validation of selected candidates for anti-cancer and immune modulation
Seon-Young Kim
/ Principal Researcher
  • Functional genomics approach to understand human cancers
Byoung-Mog Kwon
/ Principal Researcher
  • Discovery of modulator and target for antitumor-antimetastasis by chemical boiology and genomics
Kyung Chan Park
/ Senior Researcher
  • Large-scale screening and gene function study for identification of cancer-related genes
Cho-Rok Jung
/ Principal Researcher
  • Functional analysis of genes associated with cancer and its application for therapeutics
Seon-Jin Lee
/ Senior Researcher
  • Functional study of novel targeting molecule in cancer and microenvrionment
Jung-Ae Kim
/ Senior Researcher
  • Histone modifications involved in cancer progression
Mirang Kim
/ Senior Researcher
  • Epigenetic alterations during human carcinogenesis, Epigenetic mechanisms in stem cell differentiation
Keun Hur
/ Senior Researcher
  • Cancer metastasis and Epigenetics
Bo Kyung Kim
/ Researcher
  • Study of regulation mechanism and validation of therapeutic target
Hyun Seung Ban
/ Researcher
  • Chemical biology for cancer research
Hyun-Soo Cho
/ Researcher
  • Study developing a new cancer therapy targeting histone methyltransferase and demethylase
Jung-Hwa Lim
/ Researcher
  • Identification and validation of target for cancer therapy and developing UCP inhibitors as anticancer therapeutics
research areas
  • Establishment of a functional and chemical genomics research infrastructure and technology platforms
  • Large-scale screening and identification of disease related genes
  • Functional validation of candidate target genes and biomarkers for therapeutics and diagnostics development
  • Development of tools and strategies for modulating therapeutic targets and monitoring biomarkers Development of a diagnostic assay system
  • Production and application of antibodies for functional analysis of novel genes
Achievements
  • The anti-apoptotic mechanism of human TIPRL in HCC
    • Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is one of the most promising of the apoptosis-based antitumor agents. However, many cancer cells remain resistant to TRAIL-induced apoptosis, and the resistance mechanism is not clear. We report that human TIP41 is a novel anti-apoptotic target that causes cancer cells to develop resistance to TRAIL. Human TIP41, which is overexpressed in liver and lung cancer cells, interacted with MKK7 and PP2Ac to promote their dephosphorylation. A knockdown of TIP41 combined with TRAIL sustained MKK7/JNK activation and induced apoptotic cell death. These results suggest that human TIP41 may be a useful target for the development of future therapeutic strategies for TRAIL resistance.
  • E2-EPF UCP as a target for therapeutic angiogenesis.
    • E2-EPF ubiquitin carrier protein (UCP) stabilizes hypoxia-inducible factor-1α (HIF-1α) inducing ischemic vascular responses. We investigated the effect of UCP gene transfer on therapeutic angiogenesis. Adenovirus-encoded UCP (Ad-F-UCP) and conditioned media from UCP-overexpressing cells increased the expression of angiogenic factors threby promotes vascularization in cells and mice. In mouse hindlimb ischemia model (N = 30/group), only 23% of the mice injected with Ad-F-UCP showed autoamputation after 21 days of treatment. UCP gene transfer prevented muscle degeneration and autoamputation of ischemic limb. The results suggest that E2-EPF UCP may be a target for therapeutic angiogenesis.
  • Novel epigenetic target in gastric cancer
    • In the current study, we newly found that the promoter of the gene coiled-coil domain-containing 67 (CCDC67) was to be frequently methylated in gastric cancer cell lines and in primary gastric tumors. Treatment with 5-aza-2'-deoxycytidine and/or trichostatin A restored CCDC67 expression in down-regulated cell lines. Pyrosequencing analysis of 150 paired primary gastric cancer samples revealed that promoter CpG methylation was increased in 74% of tested tumors compared with paired adjacent normal tissues, and this hypermethylation correlated significantly with down-regulation of CCDC67. Stable transfection of a CCDC67 gene in gastric cancer cells inhibited adhesion-dependent and -independent colony formation, and CCDC67 expression suppressed tumorigenesis in nude mice, suggesting that CCDC67 is a putative tumor suppressor gene that is silenced in gastric cancers by promoter CpG methylation and that it may play an important role in cell signaling and migration related to tumorigenesis.
  • Function of PRL-3 in cancer metastasis
    • It was found that Phosphatase of regenerating liver (PRL-3) induced MMP-7 through oncogenic pathways including PI3K/AKT and ERK and that there is a relationship between the expression of PRL-3 and MMP-7 in human tumor cell lines. These results suggest that the migration and invasion of PRL-3- expressing CRC cells depends primarily on the expression of MMP-7.
Selected Publications
  • Misun Won (Corresponding) Angewandte Chem. 52(39):10286-9.
    • Identification of malate dehydrogenase 2 as a target protein of the HIF-1 inhibitor LW6 using chemical probes
  • Kyung-Sook Chung (Corresponding) Apoptosis. 18(7):870-81.
    • Reactive oxygen species-mediated activation of the Akt/ASK1/p38 signaling cascade and p21Cip1 downregulation are required for shikonin-induced apoptosis
  • Yong Sung Kim (Corresponding) British J Cancer. 108(9):1862-9.
    • Feasibility of proposed single-nucleotide polymorphisms as predictive markers for targeted regimens in metastatic colorectal cancer
  • Hee Gu Lee (Corresponding) Cell Death Dis. 4:e974.
    • Cystatin SN neutralizes the inhibitory effect of cystatin C on cathepsin B activity
  • Seon-Young Kim (Co-corresponding) Forensic Sci Int Genet. 7(1):143-50.
    • Genome-wide mRNA profiling and multiplex quantitative RT-PCR for forensic body fluid identification
  • Byoung-Mog Kwon and Dong Cho Han (Co-corresponding) J Biol Chem. 288(40):28713-26.
    • The natural compound cantharidin induces cancer cell death through inhibition of heat shock protein 70 and Bcl-2-associated athanogene domain 3 expression by blocking heat shock factor 1 binding to promoters
  • Jae Wha Kim (Co-corresponding) Oncol Rep. 30(4):1890-8.
    • NDRG2 positively regulates E-cadherin expression and prolongs overall survival in colon cancer patients
  • Seon-Young Kim and Yong Sung Kim (Co-corresponding) Carcinogenesis. 33(8):1494-501.
    • Epigenetic alteration of CCDC67 and its tumor suppressor function in gastric cancer
  • Nam-Soon Kim (Co-corresponding) Gastroenterology. 143(5):1341-51.
    • Inhibition of MKK7-JNK by the TOR signaling pathway regulator-like protein contributes to resistance of HCC cells to TRAIL-induced apoptosis
  • Dong Cho Han and Kwon BM (Co-corresponding) Int J Cancer. 131(3):E190-203.
    • Phosphatase of regenerating liver-3 promotes migration and invasion by upregulating matrix metalloproteinases-7 in human colorectal cancer cells
  • Misun Won (Co-first) J Med Chem. 55(23):10564-71.
    • Synthesis and structure-activity relationship of (E)-phenoxyacrylic amide derivatives as hypoxia-inducible factor (HIF) 1α inhibitors
  • Cho-Rok Jung and Dong Soo Im (Co-corresponding) Mol Ther. 20(4):778-87.
    • Adenovirus-mediated E2-EPF UCP gene transfer prevents autoamputation in a mouse model of hindlimb ischemia