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Title Developed multivalent protein optical center that enables detection of genes with high sensitivity mail
Date Dec 14, 2017
▲ (Top)  Mematic diagram of the process to detect genes by applying multivalent protein to gold nanowire optical center, (bottom left) result of detecting genes to attomolar level, (bottom right) result of detecting three kinds of genes simultaneously

- Successful detect genes with high sensitivity by synthesizing multivalent protein that combines only double stranded genes and allying it to gold nano wire optical sensor
- Expected to be utilized for precise disease diagnosis based on nano-bio convergence technology

Domestic researchers successfully synthesize multivalent protein that combines double stranded genes selectively and apply it to wire nano wire optical sensor to detect genes with high sensitivity. This technology is expected to contribute to the development of ** protein probe to detect various biomarkers and development of high sensitivity, high specific disease diagnosis technology.
   * Multivalent protein: multivalent protein structure where several same proteins are connected.
   ** Protein probe: protein that combines specifically with biomarker enabling detection.

The research conducted by Dr. Kang Tae-jun of Hazards Monitoring BNT Research Center of he Korea Research Institute of Bioscience & Biotechnology (KRIBB, President Chang Kyu-tae), research team led by professor Jeong Yong-won and Kim Bong-soo of Department of Chemistry, KAIST (corresponding author: Jeong Yong-won/Kang Tae-joon/Kim Bong-soo, lead author: Lee Joeong-min/ Hwang A-reum) was conducted with the support of global frontier project bio nano health guard research by the Ministry of Science and ICT and published on the online version of Angewandte Chemie International Edition, IF=11.994, a world’s renowned journal in the chemistry area. (Title of the paper: Multivalent Structure-Specific RNA Binder with Extremely Stable Target Binding but Reduced Interactions to Nonspecific RNAs)

The research team synthesized multivalent protein which combines with double stranded genes selectively for the first time through protein engineering process and found that the protein has very high coherence with double stranded genes. However, the team found problems of detecting genes as non-specific coherence with single stranded genes increases.

To solve this problem, the research team developed optimized multivalent protein which is combined with target genes specifically and does not combine with non-target genes by adjusting surface electric charge of multivalent protein developed. The team applied this multivalent protein to gold nano wire optical center and successfully detect genes to the level of *** attomolar.
   ***Attomolar concentration: very low concentration to the extent that only 6.02×105 genes exist in 1 liter of solvent.

The achievement was to detect genes which can be used as bio marker for disease diagnosis with high sensitivity by combining optimized multivalent protein with gold nano wire optical sensor. In particular, it was possible to detect many genes simultaneously confirming the possibility of this technology as a sensor for diagnosing diseases.
It means that the research team synthesized multivalent protein that combines with double stranded genes for the first time and maximized the capability of detecting genes by adjusting electric charge of protein. In addition the team could detect genes for diagnosing diseases to the attomolar concentration level by applying the protein to gold nano wire optical sensor.

Dr. Kang Tae-jun, who is responsible for the research said “the outcome of this research is to develop new protein probe that can strongly combine double stranded genes using multivalent mutual reaction and show how to reduce non-specific combination by adjusting electric charge of protein surface”. He added that “this research outcome is expected to contribute significantly to the development of protein probe which combines various biomarkers specifically and development of high sensitivity, high specific disease diagnosis technology.”